生物
支持细胞
生殖细胞
精子发生
细胞生物学
条件基因敲除
基因剔除小鼠
精子发生
基因敲除
血睾丸屏障
细胞生长
表型
基因
遗传学
内分泌学
作者
Shaomei Mo,Shen Ge,Congcong Cao,Mingxia Wang,Jie Yang,Jing Ye,Yaoting Gui,Shuiqiao Yuan,Qian Ma
标识
DOI:10.1093/biolre/ioae055
摘要
Abstract Sertoli cells (SCs) act as highly polarized testicular cells that nutritionally support multiple stages of germ cell development. However, the gene regulation network in SCs for modulating germ cell development has yet to be fully understood. In this study, we report that heterogeneous nuclear ribonucleoproteins C (hnRNPC) in SCs are essential for germ cell development and male fertility. Conditional knockout of hnRNPC in mouse SCs leads to aberrant SC proliferation, disrupted cytoskeleton of SCs, and compromised blood-testis barrier function, resulting in loss of supportive cell function and, ultimately, defective spermiogenesis in mice. Further RNA-seq analyses revealed these phenotypes are likely caused by the dysregulated genes in hnRNPC-deficient SCs related to cell adhesion, cell proliferation, and apoptotic process. In conclusion, this study demonstrates that hnRNPC plays a critical role in SCs for maintaining the function of SCs and sustaining steady-state spermatogenesis in mice.
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