赫拉
纳米载体
癌细胞
阿霉素
膜联蛋白
细胞凋亡
双金属片
药物输送
磷脂酰丝氨酸
化学
材料科学
生物物理学
核化学
纳米技术
癌症
细胞
生物化学
有机化学
膜
医学
生物
化疗
金属
外科
内科学
磷脂
作者
Arunachalam Chinnathambi,Sulaiman Ali Alharbi,M. Velmurugan,Sridhar Muthusami,Palanisamy Arulselvan
标识
DOI:10.1016/j.procbio.2023.12.007
摘要
An environmentally friendly preparation technique was used to explore the mechanism of action of hybrid hydrophilic bimetallic gold-platinum (Au@Pt-NPs) against human cancer cell lines, followed by conjugation with doxorubicin (DOX). The synthesized DOX-loaded Au@Pt-NPs (DOX/Au@Pt-NPs) were examined using transmission electron microscopy, X-ray diffraction, and UV-vis spectroscopy to determine their physicochemical characteristics. Using a straightforward incubation method, phytochemicals-mediated capping efficiency and loading of DOX onto the Au@Pt-NPs surface (84%) was demonstrated by FT-IR spectroscopy. Studies on the release kinetics of DOX under acidic tumor conditions revealed a pH-regulated dependence response. According to the in vitro anticancer assessment, human cancer cells treated with DOX-loaded Au@Pt-NPs saw a 3-fold increase in apoptosis compared to DOX treatment alone. Additionally, the DOX/Au@Pt-NPs nanocarrier demonstrated a time-released phosphatidylserine exposure, an apoptosis induction marker, and a cell-specific reaction against HeLa and HepG2 cells, according to luminescent results from a real-time, bioluminescent recombinant annexin V test. According to the results, the manufacturing process is simple, and the hydrophilic Au@Pt-NPs were successful in DOX delivery and loading against human cancer cell lines, indicating their prospective as a supplementary cancer therapy.
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