Strategies for multimodal spatiotemporal profiling of phosphorylation in cilia biology

ABSTRACT This Opinion piece highlights recent advances in technical approaches to dissect the mechanisms coupling cell cycle and ciliogenesis during G0/quiescence and the importance of transient post-translational modifications (PTMs) as dynamic regulators of these processes. We discuss the latest technologies enabling real-time monitoring of context-dependent phosphoproteomics and emerging concepts in PTM-driven control of ciliary function. Additionally, we outline major unanswered questions and propose future research directions. A better understanding of G0 regulatory pathways might both spur the development of clinical interventions for human cilia-linked pathologies and set the stage for future research linking cell quiescence to cancer and tissue regeneration.