Muricauda meishanensis sp. nov., a facultative anaerobic bacterium belonging to the family Flavobacteriaceae isolated from marine sediment in the East China Sea

A facultative anaerobic, Gram-strain-negative, rod-shaped bacterium (strain NBU2970 T ) was isolated by using modified ichip in situ cultivation from a marine sediment sample collected from Meishan Island in the East China Sea. Strain NBU2970 T grew optimally at 37 °C, with a NaCl concentration of 2.0 % (w/v) and at pH 7.0. The 16S rRNA gene sequence analyses revealed that strain NBU2970 T represents a novel species with the genus Muricauda , sharing highest sequence identities with Muricauda beolgyonensis BB-My12 T (96.1 %), Muricauda alvinocaridis SCR12 T (96.0 %), Muricauda taeanensis 105 T (96.0 %) and Muricauda ruestringensis B1 T (95.6 %). Phylogenetic analyses also indicated that strain NBU2970 T clustered with the genus Muricauda and was closely related to M. beolgyonensis BB-My12 T and M. ruestringensis B1 T . The draft genome sequence of strain NBU2970 T was composed of six contigs with a size of 3.2 Mbp, containing 3045 protein-coding genes and 38 RNA genes. The DNA G+C content was 43.8 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain NBU2970 T and related species of the genus Muricauda were well below the threshold limit for prokaryotic species delineation. The major cellular fatty acids were iso-C 15 : 0 , iso-C 15 : 1 G and iso-C 17 : 0 3-OH. The only respiratory quinone was MK-6. The major polar lipid was phosphatidylethanolamine. Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU2970 T is considered to be a representative of a novel species in the genus Muricauda , for which the name Muricauda meishanensis sp. nov. is proposed. The type strain is NBU2970 T (=KCTC 82915 T =MCCC 1K06394 T ).