StarBright Dyes: New Tools for Flow Cytometry

Abstract StarBright Dyes are fluorescent nanoparticles, with superior brightness over conventional flow cytometry dyes. They have narrow excitation and emission peaks, hence low spillover, spreading and compensation, providing greater flexibility when building multiplex panels in flow cytometry. StarBright Dyes have been conjugated to numerous antibodies against surface markers found on human and mouse cells. High stability and batch consistency have been confirmed using the 5-laser and 27-detector ZE5 Cell Analyzer. The antibody conjugates also showed high reagent compatibilities, without a need for special cell staining buffers even in multiplex panels with additional conjugates and can be pre-mixed and used at a later date. Furthermore, they are able to reliably identify positive cells regardless of the staining buffer and conditions used, providing flexibility and helping researchers avoid false negatives. StarBright Dyes are new valuable tools to fulfill unmet needs in immunology-based flow cytometry.