Extraction Optimization and Bioactivity of Polysaccharides from Ganoderma leucocontextum Spores

Background: Oxidative stress is associated with the occurrence and progress of aging. Natural polysaccharides have attracted considerable attention in the field of antioxidants and anti-aging products due to their superior biological activity and low toxicity. Ganoderma leucocontextum is primarily found in the Tibetan plateau region and is classified as a subspecies of Ganoderma. Known as the famous white Ganoderma, it is a precious food and medicine that has potent biological activity, including antitumor, hypoglycemic, and immune regulation. Since available resources are limited, there are few studies on the spore of Ganoderma leucocontextum. Methods: In this work, a polysaccharide (named GLSP) was extracted from the spore of Ganoderma leucocontextum using a fast, simple, efficient, and environmentally friendly extraction process: the three-phase partitioning (TPP) method. Results: The extraction condition was optimized under the Box-Behnken design (BBD): ratio of the solute to the solvent, 1:21.126 (w/v); (NH4)2SO4 concentration, 30% (w/v); ratio of the slurry to tert-butanol, 1:1.945 (v/v); and shaking temperature, 54.136 °C. Furthermore, a polysaccharide termed GLSP-A1 was purified from GLSP by column chromatography. The basic physicochemical properties were analyzed by molecular weight, Fourier transform infrared spectroscopy, monosaccharide composition, and scanning electron microscopy. Conclusions: GLSP-A1 down-regulated the expression of the pro-inflammation cytokines interleukin-6 and interleukin-1β, indicating favorable in vitro anti-inflammatory properties. In vivo, the effect of GLSP-A1 on aging was examined using the Caenorhabditis model. The results showed that GLSP-A1 reduced reactive oxygen species levels and lipofuscin accumulation. In general, these findings improve our understanding of the chemical content and bioactivity of a polysaccharide from Ganoderma leucocontextum spore and highlight the possibility of GLSP-A1 being utilized in dietary supplements for its anti-aging properties.