化学
溶剂
蛋壳膜
圆二色性
Zeta电位
膜
结晶学
降水
分子间力
蛋白质二级结构
盐(化学)
化学工程
粒径
生物物理学
分子
有机化学
生物化学
物理化学
生物
物理
气象学
纳米颗粒
工程类
作者
Xinhua Liang,Honglian Cong,Xinhua Liang
摘要
ABSTRACT This study investigated the effects of different solvent systems on the microstructure, roughness, and secondary structure of soluble eggshell membrane proteins (SEPs). The solvent system of CaCl 2 /C 2 H 5 OH/H 2 O produced tiny holes on the surface of SEP, and LiBr resulted in the formation of long holes. The saline solution increased the diameter of the protein fiber, the particle size of the solution, and the surface roughness of regenerated SEP films, mainly due to the enhanced intermolecular aggregation and precipitation. Zeta potential measurements indicated salts decreased the negative values and reduced the stability of the SEP solution. The different solutions showed similar circular dichroism waveforms. The peak intensity decreased at the positive and negative peaks, indicating that the triple helix structure of collagen was denatured to different degrees. Besides, the addition of salts decreased the content of α‐helices and the β‐turns and increased the content of β‐sheets and random coils, indicating an increase in the disordered structure of the protein. This study contributes to a deeper understanding of the structural and functional relationships of eggshell membrane proteins, providing a vital basis for developing novel, eco‐friendly, and multifunctional protein materials.
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