糖酵解
胆碱激酶
生物
新陈代谢
酶
胆碱
生物化学
磷脂
细胞生物学
泛素连接酶
化学
泛素
磷脂酰胆碱
膜
基因
作者
Qingxia Ma,Hongfei Jiang,Leina Ma,Guiqiu Zhao,Qianqian Xu,Dong Guo,Ningning He,Hao Líu,Zhaoyuan Meng,Juanjuan Liu,Lei Zhu,Qian Lin,Xiaolin Wu,Min Li,Shudi Luo,Jing Fang,Zhimin Lu
标识
DOI:10.1073/pnas.2209435120
摘要
Aberrantly upregulated choline phospholipid metabolism is a novel emerging hallmark of cancer, and choline kinase α (CHKα), a key enzyme for phosphatidylcholine production, is overexpressed in many types of human cancer through undefined mechanisms. Here, we demonstrate that the expression levels of the glycolytic enzyme enolase-1 (ENO1) are positively correlated with CHKα expression levels in human glioblastoma specimens and that ENO1 tightly governs CHKα expression via posttranslational regulation. Mechanistically, we reveal that both ENO1 and the ubiquitin E3 ligase TRIM25 are associated with CHKα. Highly expressed ENO1 in tumor cells binds to I199/F200 of CHKα, thereby abrogating the interaction between CHKα and TRIM25. This abrogation leads to the inhibition of TRIM25-mediated polyubiquitylation of CHKα at K195, increased stability of CHKα, enhanced choline metabolism in glioblastoma cells, and accelerated brain tumor growth. In addition, the expression levels of both ENO1 and CHKα are associated with poor prognosis in glioblastoma patients. These findings highlight a critical moonlighting function of ENO1 in choline phospholipid metabolism and provide unprecedented insight into the integrated regulation of cancer metabolism by crosstalk between glycolytic and lipidic enzymes.
科研通智能强力驱动
Strongly Powered by AbleSci AI