AB1070 DIVERSITY ANALYSIS OF INTESTINAL FLORA IN PATIENTS WITH BEHCET’S DISEASE

Background: Behçet’s disease (BD) may be regarded as a polygenic autoinflammatory disease although adaptive immune system has also been implicated in pathogenesis. The specific components of the microbiota with BD that affect the host response leading to disease remain unknown. Regulation of intestinal microbiota would be able to provide new strategy and target for the treatment of BD. Objectives: To study the diversity and the intestinal flora of intestinal microbes in patients with BD and further provide new ideas for clinical treatment. Methods: The stool specimens of 13 BD patients were analyzed at the level of the Phylum, family and genus, and compared with that of 50 healthy controls(HC). Results: Compared with controls, the abundance of intestinal microbiota in patients with BD was significantly different.At the level of phylum, the abundance of Firmicutes was significantly reduced in BD patients compared with that of HC(P<0.05)(fig. 1).At the genus level, in the BD group, the abundance of Klebsiella is significantly increased (P<0.05), but the abundance of Clostridium IV, Lachnospiracea incertae sedis, Anaerostipes and Megasphaera were significantly lower than that of healthy controls. (P<0.05)(fig. 2). Table 1. Demographic and Clinical Data of Patients Patient Demographics Phenotype Laboratory Prior Diagnosis(Dx) and Treatment 1 Age Onset: 63 Age at YAOS Dx: 66 Gender: F Caucasian: Yes Fever: High grade, lasting up to 36 hrs Skin: Patchy erythema on face, lasting up to 2 wks Arthritis: Knee and ankle GI: Abd pain, non-bloody diarrhea. GI workup: negative for IBD Sicca: Yes Asthma: Yes Family history : No ESR)/CRP: Normal MEFV: Neg 6-gene panel: positive for NOD2 IVS8 + 158, R702W, heterozygous FM F: Yes Colchicine(col): Transient response 2 Age Onset: 7 Age at YAOS Dx: 49 Gender: F Caucasian: Yes Fever: High grade Skin: Patchy erythema on face, chest, abd, limbs, lasting up to 6 wks Arthritis: Knee, ankle and toe GI: Abd pain, nonbloody diarrhea, lasting up to 4 d. GI workup: neg for IBD Sicca: Yes Asthma: Yes Family history : Yes ESR/CRP: Normal MEFV: Neg 6-gene panel: positive for NOD2 IVS8 + 158, heterozygous FMF : Yes Col: Good response 3 Age Onset: 15 Age at YAOS Dx: 35 Gender: F Caucasian: Yes Fever: Low grade Skin: Patchy erythema on arms and legs, lasting a few d Arthritis: Knee and shoulder GI: Abd pain with nausea and vomiting, lasting a few hrs. GI workup: neg for IBD Sicca: No Asthma: No Family history : No ESR/CRP: Normal MEFV: Neg 6-gene panel: positive for NOD2 IVS8 + 158, 1007fs, heterozygous FMF: Yes Col: Minimal response Figure 1. the differences between patients with BD and normal healthy adults were compared at the level of the phylum Figure 2. the differences between patients with BD and normal healthy adults were compared at the level of the genus Conclusion: The diversity and balance of bacterial community in intestinal microecological environment of patients with BD are significantly different from the healthy control. Intestinal microbiota disorder may be related to the pathogenesis of BD, which might provide theoretical foundation for the regulation of intestinal flora for disease intervention. Disclosure of Interests: None declared