Early life γδ T cell activation enforces intestinal barrier integrity during intergenerational C. difficile colonization

SUMMARY Early postnatal life is a highly dynamic period in which the intestine is inundated with billions of microbes that not only battle for colonization, but also strongly influence immune system function and long-term health. This brief period of development provides a window of opportunity to imprint resilience against disease, yet the intercellular dialogue that dictates tissue protection are only beginning to be discovered. By performing a kinetic analysis of immune cell activation in the colon of mice from early life to adulthood, we identified transient activation of a microbiota-dependent type 3 immune response during the weaning period. This response was characterized by a selective increase in IL-17 production by fetal-derived γδ T cells that occurred in an IL-1 receptor-dependent, cell-intrinsic manner. The subsequent differentiation of IL-10 producing Rorγt+ T regulatory cells extinguished IL-17 production by γδ T cells and prevented immunopathology. Microbial gain-of-function approaches determined that colonic γδ T cell activation occurred in response to passive acquisition of C. difficile at birth. In turn, IL-17 limited C. difficile growth and intestinal barrier breach by the commensal microbiota. Collectively, our results reveal how an agile immune cell network meets the demands of a maturing microbiota to support gut health during early life. Highlights - Colonic γδ17 T cells are activated during early life in a microbiota-specific manner - Fetal-derived γδ17 T cells require IL-1 signals for activation - Passive acquisition of C.difficile induces γδ17 T cell activation - IL-17 limits C. difficile growth and systemic dissemination of commensals