化学
色谱法
诺比林
电喷雾电离
黄酮类
橙皮苷
串联质谱法
甲酸
选择性反应监测
质谱法
电喷雾
检出限
类黄酮
有机化学
医学
替代医学
病理
抗氧化剂
作者
Haifang Chen,Wugang Zhang,Yuan Jie,Yangang Li,Shilin Yang,Wenge Yang
标识
DOI:10.1016/j.jpba.2011.10.013
摘要
The major lipid-soluble constituents in Fructus aurantii (zhiqiao) and Fructus aurantii immaturus (zhishi) are polymethoxylated flavones (PMFs) and coumarins. In the present study, a high-performance liquid chromatography with electrospray ionization tandem mass spectrometry method was developed to quantify PMFs (nobiletin, tangeretin, 5-hydroxy-6,7,8,4′-tetramethoxyflavone, and natsudaidai) and coumarins (marmin, meranzin hydrate, and auraptene) simultaneously. PMFs and coumarins were detected by electrospray ionization tandem mass spectrometry in positive ion mode and quantified with multiple reaction monitor. Samples were separated on a Diamonsil C18 (150 mm × 4.6 mm, 5 μm) column using acetonitrile and formic acid–water solution as a mobile phase in gradient mode with a flow rate at 0.5 mL/min. All calibration curves showed good linearity (r2 > 0.9977) within the test ranges. Variations of the intraday and interday precisions were less than 4.07%. The recoveries of the components were within the range of 95.79%–105.04% and the relative standard deviations were less than 3.82%. The method developed was validated with acceptable accuracy, precision, and extraction recoveries and can be applied for the identification and quantification of four PMFs and three coumarins in citrus herbs.
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