化学
亲和层析
抗体
色谱法
蛋白质A
串联亲和纯化
生物化学
酶
生物
免疫学
作者
Sophia Hober,Karin Nord,Martin Linhult
标识
DOI:10.1016/j.jchromb.2006.09.030
摘要
Staphylococcal protein A (SPA) is one of the first discovered immunoglobulin binding molecules and has been extensively studied during the past decades. Due to its affinity to immunoglobulins, SPA has found widespread use as a tool in the detection and purification of antibodies and the molecule has been further developed to one of the most employed affinity purification systems. Interestingly, a minimized SPA derivative has been constructed and a domain originating from SPA has been improved to withstand the harsh environment employed in industrial purifications. This review will focus on the development of different affinity molecules and matrices for usage in antibody purification.
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