Hydrolysis at Arginylproline in Polypeptides by Clostridiopeptidase B

Clostridiopeptidase B, a sulfhydryl protease from Clostridium histolyticum, hydrolyzes the arginylproline bond in the synthetic polypeptide methionyllysyl-bradykinin. Hydrolysis also occurs at a reduced rate at the lysylarginine bond, further delineating the environment necessary for the minor proteolysis seen with this enzyme at lysine residues. The specificity of clostridiopeptidase B differs from trypsin, which hydrolyzes this synthetic polypeptide only at the lysylarginine bond.