细胞凋亡
细胞毒性T细胞
三阴性乳腺癌
细胞毒性
细胞培养
化学
癌细胞
癌症研究
程序性细胞死亡
细胞
体内
MCF-7型
药理学
作者
Ideli Zanesco-Fontes,Ana Carolina Lopes Silva,Patrícia Bento da Silva,Jonatas Lobato Duarte,Leonardo Delello Di Filippo,Marlus Chorilli,Márcia Regina Cominetti,Ana Carolina Baptista Moreno Martin
出处
期刊:Aaps Pharmscitech
[Springer Nature]
日期:2021-05-18
卷期号:22 (5)
被引量:4
标识
DOI:10.1208/s12249-021-02006-w
摘要
The apoptotic, cytotoxic, and cytostatic activities for [10]-gingerol in triple-negative breast cancer cells (TNBCs) were already reported. However, despite these important antitumor activities, the compound has the disadvantage to have a hydrophobic characteristic, hindering in vivo administration. To surpass this issue, in this study we have created a [10]-gingerol-loaded nanoemulsion (10GNE) in order to increase the stability and solubility of the compound. The nanoemulsion was characterized and tested for its cytotoxic, cytostatic, and apoptotic effects on a panel of murine and human TNBC cell lines, as well as non-tumor cells, and compared with a [10]-gingerol-free nanoemulsion (NE) and with [10]-gingerol itself. Except for the murine 4T1.13 cell line, the IC50 of the free 10G molecule, after 72 h of incubation, was higher in all cell lines tested, both murine and human, demonstrating therefore the efficacy of the 10GNE regarding cytotoxicity. In murine tumor cells, 60 μM 10GNE was able to arrest cell cycle at sub-G0 phase and induce apoptosis, leading to 48% and 78% of total cell death in 4T1.13 and 4T1Br4 murine tumor cells, respectively. This represents an improvement compared to 10G-free molecule that only induced 74% of total apoptosis at 100 μM in 4T1Br4 cells. Taken together, our results show that nanoformulation preserved the [10]-gingerol cytotoxic and cytostatic properties and improved its apoptotic function on murine TNBC cell lines. These data open new perspectives to a more suitable drug-delivery approach for [10]-gingerol for TNBC treatment that should be further demonstrated using in vivo assays.
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