Highly selective detection of H2O2 through a non-oxidative mechanism has been achieved by desulfonylation of 1 to the corresponding fluorescein. This approach has been used for the intracellular formation of H2O2 in algal cells by using the acetyl derivatives of 1 as fluorescent probes. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2004/z52381_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.