To establish the rapid determination method of imidacloprid residue in rice by high performance liquid chromatograph(HPLC).Imidacloprid residue in rice was extracted with eddy-oscillat method and purified through a florisil column,detected by HPLC at 269 nm.The average recovery rate and RSD were 81.90%-87.80% and 3.24%-4.51% respectively,the detection limits of imidacloprid was 0.001 6 mg/kg.The results showed that the accuracy,precision and sensitivity of the method met the requiremeats of residue analysis of pesticide.