Research on the Construction of the Recombinant Adenovirus of PRRSV GP5 and GP4 Protein and Its Immune Characteristics

[Objective] What the corresponding encoded protein of the PRRSV protein GP5 and GP4 could be expressed in same vector,respectively was achieved and the advantage role of protein GP5 in humoral immunity and protein GP4 in signal transduction was played.[Method] The gene GP5 and GP4,amplified with the RT-PCR,was cloned into the multiple-cloning enzyme restriction site of pIRESneo vector and the neomycin phosphotransferase loci;and the expression kit of gene GP5 and GP4 with double-digested enzyme of XhoⅠ and NruⅠwas cloned into the deficiency carrier pPolyⅡ-CAV-2-E3 in the E3 Zone of type 2 of canine adenovirus,which were identified with double-digested restriction enzymeof AscⅠ and PmeⅠ.[Results] A strong humoral immune response(ELISA antibodies and neutralizing antibodies) could be induced in the recombinant adenovirus immunized mice.[Conclusion] The recombinant adenovirus had good immunogenicity,by which the basis for developing an effective subunit vaccine would be laid.