Effects of FAP on human umbilical vein endothelial cells proliferation and angiogenesis in vitro

Objective To investigate the effects of FAP on human umbilical vein endthelial cell proliferation and angiogenesis in vitro.Methods Human umbilical vein endothelial cell EA.hy926 was treated with different concentrations of FAP(0,300,600,1200 pmol/L).Cell proliferation was determined by MTT assay.Cell migration was assessed by a wound healing assay.And tube formation was studied with a three-dimensional gel model.Results Compared with the normal control group,FAP promoted endothelial cell migration and proliferation(P0.05),promoted tube formation of endothelial cell on the gel(P0.01).The effect of 600 pmol/L FAP was significantly higher than that of other groups(P0.01).Conclusion FAP can promote human umbilical vein endothelial cell proliferation,migration and angiogenesis in vitro.It provides a basic theoretical depiction of the role of FAP in angiogenesis.