双分子荧光互补
荧光
蛋白质-蛋白质相互作用
互补
体外
体内
化学
绿色荧光蛋白
生物物理学
氨基酸
黄色荧光蛋白
荧光蛋白
生物化学
细胞生物学
生物
遗传学
基因
物理
表型
量子力学
作者
Kazumasa Ohashi,Kensaku Mizuno
出处
期刊:Methods in molecular biology
日期:2014-01-01
卷期号:: 247-262
被引量:14
标识
DOI:10.1007/978-1-4939-0944-5_17
摘要
Protein–protein interactions are critical components of almost every cellular process. The bimolecular fluorescence complementation (BiFC) method has been used to detect protein–protein interactions in both living cells and cell-free systems. The BiFC method is based on the principle that a fluorescent protein is reassembled from its two complementary non-fluorescent fragments when an interaction occurs between two proteins, each one fused to each fragment. In vivo and in vitro BiFC assays, which use a new pair of split Venus fragments composed of VN210 (amino acids 1–210) and VC210 (amino acids 210–238), are useful tools to detect and quantify various protein–protein interactions (including the cofilin–actin and Ras–Raf interactions) with high specificity and low background fluorescence. Moreover, these assays can be applied to screen small-molecule inhibitors of protein–protein interactions.
科研通智能强力驱动
Strongly Powered by AbleSci AI