Laccase production by white rot fungi Pleurotus ostreatus and its optimal inducing condition

Laccase is capable of catalyzing the oxidation of a broad number of phenolic compounds and aromatic amines. The oxidized substrates by laccase can be extended in the presence of small molecular mediators. Pleurotus ostreatus 3.42 is an excellent producer of the industrially important enzyme laccase. Growth and laccase production in static cultivation are superior to that in agitated cultivation, and Nlimited culture is of benefit to laccase production. By using cellulose and peptone as carbon and nitrogen source, a higher activity level is obtained. Experiment demonstrates that ABTS (1?mmol) is the best inducer of laccase production, reaching a maximum value of 1?000?U/mL, approximately.