Progress in the Nonviral Vectors of Gene Therapy

The vectors of gene therapy include viral vector and nonviral vector. For the problems of the safety of viral vector, at present, the research of nonviral vector is important. Nonviral vectors is a newly gene delivery system which has lower-toxicant,less immune reactions, lower probability of gene integration, and non-limitation of gene insertion clip size,it also has other characteristics such as the convenience of the use and preparation, easy conservation and probation. As a normal nonviral vector, liposome was easily to be prepared and has high safety to be preserved, but it was so hardly to get the requirement of quality control. For the lower efficiency of gene delivery in vivo and problems of target, its application was limited. The research of pH-sensitive liposome and positive ion liposome increase the efficiency of delivery of liposome. And the application of the high polymer carriers and nanoparticle in the gene therapy has open up a vast range of prospects for making use of nonviral vector in clinical practice.