D-amino Acid Oxidase:Update and Review

D-amino acid oxidase(EC 1.4.3.3,DAAO)is a FAD-dependent enzyme that plays an important role in metabolism of endogenous D-amino acids in living cells.The D-amino acid oxidase(DAAO)catalyzes oxidative deamination of D-amino acids yielding hydrogen peroxide,ammonium and the corresponding α-keto acids.The recent data about this enzyme was summarized,including its physiological role and practical applications,optimization of expression,and the characteristics of some single-point mutant enzyme which designed by a rational approach.