Genetic Diversity Analysis of Nelumbo nucifera Based on SRAP Markers

A newly developed molecular marker-SRAP(sequence-related amplified polymorphism) was used to detect DNA polymorphisms among the 39 lotus cultivars.Five pair primers were used to amplify the 72 genomic DNA.A total of 101 bands were generated,88 of them were polymorphic bands,with an average of 20 marker bands per pair of primers.The dendrogram generated by UPMGA showed the genetic relationship among 39 lotus cultivars.There were obvious definition among three types of flower,seed and rhizome lotus.Flower lotus is close to seed lotus in genetic relationships,while rhizome lotus has the farthest relationships to the others.Genetic variation within cultivars was more than that among cultivars in induced flower lotus,general flower lotus and induced seed lotus,and it was over 70% and 60% in induced seed lotus and induced flower lotus,respectively.However,genetic variation within cultivars was less than that among cultivars in rhizome lotus.Genetic variation within cultivars is important to both high and steady yield and selecting more choiceness new cultivars from abroad planting cultivars and freshly cultivated cultivars.