OBJECTIVE To select the best processing method of R.emodi Wall.with the goal of decreasing the content of anthraquinones.METHODS The components of piceatannol-4-O-β-D-glucopyranoside(PGP) and total anthraquinones(TA) were chosen as index and determined by HPLC.The content changes of stilbene glycosides and total anthraquinones in Rheum emodi Wall were investigated after being processed in the ways of water steaming,water steaming after yellow wine,stir frying after vinegar and water steaming after black soybean solution.RESULTS The retention rate of PGP in Rheum emodi Wall.processed in the ways of water steaming,water steaming after yellow wine,stir frying after vinegar and water steaming after black soybean solution were 51.2%,36.8%,81.1% and 74.9%,respectively.Removing rate of TA were 53.2%,74.1%,19.9% and 15.3%,respectively.CONCLUSION Although four processing methods did not reach the optium purpose,it can provide scientific references for the study and application of Rheum emodi Wall..