Chromatography of Biomolecules

Chromatography of macromolecular biomolecules, including units and definitions, plate theory, rate theory, size exclusion (gel filtration), gel matrices for size exclusion chromatography, affinity chromatography, immobilization of affinity ligands, elution methods, determination of association constants by high performance affinity chromatography, ion-exchange chromatography, retention model for ion-exchange chromatography of polyelectrolytes.