石墨烯
化学
电化学发光
化学发光
氧化物
适体
胶体金
纳米复合材料
电极
纳米颗粒
检出限
无机化学
生物传感器
光化学
共价键
纳米技术
物理化学
材料科学
有机化学
色谱法
生物
遗传学
生物化学
作者
Yaping Dong,Ying Zhou,Jiao Wang,Jun‐Jie Zhu
标识
DOI:10.1021/acs.analchem.6b00921
摘要
In the present work, strong anodic electrogenerated chemiluminescence (ECL) of Ru(bpy)3(2+) was observed at a graphene oxide modified glassy carbon electrode (GO/GCE) in the absence of coreactants. The electrocatalytical effect of GO on the oxidation of Ru(bpy)3(2+) suggested that GO itself can act as the coreactant of Ru(bpy)3(2+) ECL, which can be used to fabricate the ECL biosensor. Thiol group terminated adenosine triphosphate (ATP) aptamer was immobilized on the GO film via DNA hybridization. When gold nanoparticles/graphene oxide (AuNPs/GO) nanocomposites were modified on the aptamer through the S-Au bond to form a sandwich-like structure, the ECL resonance energy transfer (ECL-RET) could occur between Ru(bpy)3(2+) and AuNPs/GO nanocomposites, resulting in an apparent decrease of ECL signal. After the ECL sensor was incubated in ATP solution, the AuNPs/GO nanocomposites were released from the electrode due to the specific interaction between aptamer and ATP, leading to the increased ECL signal. On the basis of these results, an ECL aptasensor was fabricated and could be used in the sensitive and selective detection of ATP in the range of 0.02-200 pM with a detection limit of 6.7 fM (S/N = 3). The proposed ECL aptasensor can be applied in the detection of ATP in real samples with satisfactory results.
科研通智能强力驱动
Strongly Powered by AbleSci AI