Nanostructure and nanotechnology promote the development of biomedicine.Due to its excellent catalysis activity and optical characteristics,gold nanoparticles have been widely used in the fields of nano-device design,nano-biotechnology,nano-biomedicine,nano-pharmacology and so on.The latest progresses on the applications of gold nanoparticles including tumor therapy,cellular imaging and drug carrier were introduced.At the same time,the importance of gold nanoparticles in biomedicine is emphasized and its biologic negative-effect is also presented.At last,the tendency of gold nanoparticles is discussed.