Determination of Octafluoropropane in Canine Exhaled Gas by GC-MS-MS

OBJECTIVE To develop a method for the determination of octafluoropropane in canine exhaled gas.To assess the exhaled elimination kinetics of octafluoropropane in anesthetized canines following intravenous administration of octafluoropropane-containing human albumin micropheres.METHODS Octafluoropropane-containing albumin microbubbles at dosages of 0.4,0.8 and 1.2 mL·kg-1 were administered intravenously to anesthetized and ventilated canines.The exhaled air samples were analyzed by gas chromatography-mass spectrum-mass spectrum(GC-MS-MS).The plarmacokinetic parameters were evaluated by WINNOLIN software and noncompartmental analyses.RESULTS The calibration curve of GC-MS-MS was linear within the concentration range of 40.92~16 368 nmol·L-1.In dose of 0.4,0.8 and 1.2 mL·kg-1,cmax were(2.837±0.25),(3.06±0.29) and(13.64±2.99)nmol·L-1 respectively;tmax were(16.67±10.53),(21.66±14.12) and(10±0.00) s,respectively.AUC were(154.19±61.83),(353.77±77.89) and(803.21±335.77) nmol·s·L-1,respectively;MRT were(96.68±1.12),(134.39±38.56) and(91.02±9.27)s,respectively.CONCLUSION The method of GC-MS-MS is accurate ,sensitive,precise and usable for OFP.