Purification and Chemical Composition of Polysaccharides from Chrysanthemum indicum

Objective:To study on the purification and chemical composition of polysaccharides from Chrysanthemum indicum.Method:Polysaccharides were extracted from C.indicum with hot water,followed by precipitating with ethanol,decolorizing with LSA-21 and removing of free protein by the method of Sevag,and finally separated into three fractions CIP-1′、CIP-2′and CIP-3′by DEAE-52 cellulose anion exchange and Sephadex G75 gel column chromatograph.Result:The relative molecular weight(Mw) of CIP-1′was estimated to be about 8,242 Da by using high-performance gel permeation chromatography(HPGPC),while that of CIP-2′was 8,383 Da and of CIP-3′was 1.920 1×103 Da.According to GC analysis,CIP-1′was composed of six kinds of monosaccharides,namely rhamnose,arabinose,xylose,mannose,glucose and galactose in the molar ratio of 5.44:16.40:20.35:3.53:15.43:21.46,following that CIP-2′was composed of the same kinds of monosaccharides in the molar ration of 8.47:13.17:2.43:2.75:14.51:15.76.However,CIP-3′was composed of rhamnose,arabinose,mannose,glucose and galactose in a molar ratio of 17.46:15.88:18.90:63.76:36.44.The FT-IR spectrum of the three also indicated typical characteristics of polysaccharides.Conclusion:This study provided experimental basis for the development of polysaccharide resources from C.indicum.