Separation of Amino Acids by Capillary Zone Electrophoresis Using 9-(2-Carbazole) Ethyl Chloroformate as Derivatization Agent

A simple and rapid method for the separation of amino acids by capillary zone electrophoresis using 9(2carbazole)ethyl chloroformate (CEOC) as derivatization agent was developed. The effect of some key factors such as pH, temperature and concentration of buffer, on the separation of amino acid derivatives was investigated. 12 amino acids were separated with satisfactory results.