Agrobacterium-mediated transformation of tomato with the ICE1 transcription factor gene

ICE1 genes play a very important role in plants in cold conditions.To improve the cold resistance of tomato, the ICE1 gene of Arabidopsis thaliana was used to construct the plant expression vector p3301-ICE1, and was overexpressed in tomato through Agrobacterium-mediated transformation.Five strains of resistant plants were obtained.PCR and half-quantitative results showed that the ICE1 gene was transferred to tomato; three strains tested positive.After lowtemperature stress treatment, praline content and peroxide and catalase 598 ©FUNPEC-RP www.funpecrp.com.