Influence of Spred2 on apoptosis of human hepatocellular carcinoma cells

Objective To observe the effect of Spred2 on apoptosis of human hepatocellular carcinoma cells. Methods HepG2 cells were transfected with pcDNA3. 0 and pcDNA3. 0-Spred2 for the morphologic change of HepG2 to be observed. Cells were stained with annexin Ⅴ conjugated to fluorescein isothiocyanate(annexin Ⅴ-FITC) and propidium iodide(PI/7-AAD) . The stained cells were analyzed on a flow cytometer using the Cellquest program. The expression level of Spred2 was examined by Western blot. Results Spred2 over-expression induced HepG2 cells apoptosis and sensitized cells to 5-FU induced HepG2 cells apoptosis. Conclusions Spred2 can regulate HepG2 cellular apoptosis.