Radiosensitivity of Hepatocarcinoma Cell Lines HepG2 and BEL-7402

Objective To evaluate the radiosensitivity of hepatocarcinoma cells HepG2 and BEL-7402 in vitro.Methods Using cell culture of human being hepatocarcinoma cell lines HepG2 and BEL-7402,we measured their surviving fraction by clonogenic assay and MTT after irradiated by 6MV X ray with doses ranging 0～10 Gy.Radiosensitivity index,D 0,was calculated.Results The D 0 of HepG2 was 1.5 Gy,SF 2(0.45±0.05).The D 0 of BEL-7402 was 1.6 Gy,SF 2(0.63±0.05).There was a good correlation between the results derived from MTT and clonogenic assay(γ=0.946, P 0.05).Conclusion The radiosensitivity of HepG2 is rather high compared with BEL-7402.MTT is a quick way to measure radiosensitivity of cells in vitro instead of clonogenic assay.