Determination of fibrinolytic activity in the plasma of several animals by means of chromogenic substrate

The chromogenic substrate S-2251 (H-D-Val-Leu-pNA), a high specific and sensitive for SK-plasminogen complex and plasmin in man, was used for determination of plasminogen and anti-plasmin in various species of laboratory animals. Plasma plasminogen of the monkey, dog, rat and rabbit showed significant species differences in activation by the streptokinase; those of the rat and rabbit were not activated by the streptokinase. Therefore the urokinase was better for activation of the plasminogens of these animals. The anti-plasmin activity was highest in the rat, and followed in an order of the monkey, rabbit, man and dog.Using the method for determination of the plasminogen activity established in the above studies, influence of alloxan-induced hyperglycemia on the plasminogen activity in the rat was studied and found that it decreased by the alloxan treatment. No change was observed in the anti-plasmin and plasmin activities.