Chemical constituents in Alpinia oxyphylla seed

Using column chromatography,MS and NMR techniques,compound Ⅰ-Ⅵ were isolated and identified from the ethyl acetate fraction of 80% ethanol extract of Alpinia oxyphylla Miq.seed,and compound Ⅶ-Ⅸ from the petroleum ether fraction.Nine compounds are as follows: yakuchinone A(Ⅰ),yakuchinone B(Ⅱ),tectochrysin(Ⅲ),4-methoxy-1,2-dihydrocyclobutabenzene(Ⅳ),protocatechuic acid(Ⅴ),β-daucosterol(Ⅵ),sitosteryl palmitate(Ⅶ),palmitic acid(Ⅷ) and stigmasterol(Ⅸ).In which,compound Ⅳ is a new natural compound,and compound Ⅶ,Ⅷ and Ⅸ are isolated from A.oxyphylla for the first time.