Polyester fabric was dyed with indigo naturalis and factors influenced on dyeing effect were studied.The results showed that the optimum dyeing conditions were pH value 4~5,the dyeing temperature 120~130 ℃ and dyeing time 30 minutes.Polyester could be dyed by indigo naturalis at 100 ℃ in the presence of carrier.The polyester fabric dyed with indigo naturalis has high washing fastness and rubbing fastness.