Bcl2-siRNA increases sensitivity of human lung adenocarcinoma A549/DDP cells to cisplatin

Objective To study the effect of Bcl2-siRNA on sensitivity of cisplatin-resistant human lung adenocarcinoma A549/DDP cells to cisplatin.Methods Forty-eight hours after Bcl2-siRNA was transfected with cisplatin-resistant human lung adenocarcinoma A549/DDP cells,a fresh cisplatin solution was prepared by adding different concentrations of cisplatin(0μg/ml,3μg/ml,6μg/ml,9μg/ml,12μg/ml,15μg/ml) into A549/DDP cells to measure their growth-inhibition rate by CCK-8 assay.Expression of Bcl-2 mRNA and protein in A549/DDP cells-transfected Bcl2-siRNA was detected by Real time RT-PCR and Western blot,respectively.Results The expression of Bcl-2 mRNA and protein in A549/DDP cells-transfected Bcl2-siRNA decreased 92.6%(P0.05) while the sensitivity of A549/DDP cells to cisplatin increased 57.8%(P0.05).Conclusion Bcl2-siRNA can decrease the expression of Bcl-2 mRNA and protein in A549/DDP cells,and increase the sensitivity of A549/DDP cells to cisplatin.