Aim To get law of effects of inner supports on liquid film controlled mass transfer in rotating packed bed (RPB) and further provide theoretical support for industrialization of RPB. Methods The working system of the experiment is desorption of O\-2 in water by N\-2, which is a typical process of liquid film controlled mass transfer. The different effects of inner support on liquid film controlled mass transfer are taken through Number of Transfer Unit(NTU). Results The influences of rotating speed and liquid flow rate on Number of Transfer Unit (NTU) has been investigated. Furthermore, in rotating packed bed with the inner supports, the effects of shape, thickness, the voidage and the position of the inner supports on Number of Transfer Unit(NTU) have been investigated too. Conclusion The results of the experiment have guiding significance on use and exploitation of RPB.