人参
人参皂甙
三七
五加科
次生代谢物
生物
传统医学
化学
内生菌
茉莉酸甲酯
植物
生物化学
医学
基因
病理
替代医学
作者
Xiaolin Song,Hao Wu,Zhenhao Yin,Mei-Lan Lian,Chengri Yin
出处
期刊:Molecules
[MDPI AG]
日期:2017-05-23
卷期号:22 (6): 837-837
被引量:56
标识
DOI:10.3390/molecules22060837
摘要
Ginsenoside is the most important secondary metabolite of ginseng. Natural sources of wild ginseng have been overexploited. Although root culture could reduce the length of the growth cycle of ginseng, the number of ginsenosides is fewer and their contents are lower in adventitious roots of ginseng than that in ginseng cultivated in the field. In this study, we investigated the effects of endophytic bacterial elicitors on biomass and ginsenoside production in adventitious roots cultures of Panax ginseng. Endophyte LB 5-3 as an elicitor could increase biomass and ginsenoside accumulation in ginseng adventitious root culture. After 6 days elicitation with a 10.0 mL of strain LB 5-3, the content of total ginsenoside was 2.026 mg g−1 which was four times more than that in unchallenged roots. The combination of methyl jasmonate and strain LB 5-3 had a negative effect on ginseng adventitious root growth and ginsenoside production. The genomic DNA of strain LB 5-3 was sequenced, and was found to be most closely related to Bacillus altitudinis (KX230132.1). The challenged ginseng adventitious root extracts exerted inhibitory effect against the HepG2 cells, which IC50 value was 0.94 mg mL−1.
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