金黄色葡萄球菌
大肠杆菌
甲苯胺酮
抗菌活性
自愈水凝胶
化学
核化学
响应面法
光动力疗法
微生物学
材料科学
色谱法
细菌
有机化学
生物
生物化学
基因
遗传学
作者
Hui Liang,Jialin Xu,Yunfeng Liu,Jianxing Zhang,Wei Peng,Jun Yan,Zelin Li,Qingyong Li
标识
DOI:10.1016/j.jphotobiol.2017.06.019
摘要
Photodynamic therapy with toluidine blue O (TBO) hydrogel exhibits antibacterial activity against Staphylococcus aureus and Escherichia coli in this paper. The response surface methodology is employed to optimize formulations for antibacterial activity. The optimal formulations are carbomer concentration of 3% (w/v), TBO concentration of 0.1mg/mL and the quality ratio of NaOH and carbomer of 0.4 (w/w). Under the optimized formulations, the log-transformed of CFUmL-1 on the Staphylococcus aureus and Escherichia coli are 0.84 and 1.26 (the log-transformed of CFUmL-1 of negative control groups on the Staphylococcus aureus and Escherichia coli are 8.21 and 8.47), respectively. In comparison with photodynamic therapy with TBO aqueous solution, the proposed formulations provide a much stronger antibacterial activity against Staphylococcus aureus and Escherichia coli. TBO hydrogels are stable during 6weeks at three different temperatures (4, 25 and 40°C) with respect to no change of color, transparency, pH and viscosity. 50% and 68.26% of TBO are released from carbomer hydrogel after 4h and 24h, respectively. TBO hydrogel alone or light alone (630nm) treatment is incapable of showing antibacterial activity against Staphylococcus aureus and Escherichia coli. Therefore, photodynamic therapy with the novel optimized TBO hydrogel formulations is a promising treatment strategy for periodontitis.
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