化学
荧光
硫化氢
肝细胞癌
双光子激发显微术
癌症研究
光学
有机化学
硫黄
物理
生物
作者
Yani Liu,Yani Liu,Xueao Wang,Zheng Li,Liping Chen,Xiaoyan Cai,Zhi-Gang Sun,Wei Cheng,Xiangjie Luo,Hai‐Liang Zhu,Yong Qian,Yong Qian,Yong Qian
标识
DOI:10.1021/acs.analchem.4c00061
摘要
Hydrogen sulfide (H2S), a critical gas signaling molecule, and N-acetyltransferase 2 (NAT2), a key enzyme in drug metabolism, are both known active biomarkers for liver function. However, the interactions and effects of H2S and NAT2 in living cells or lesion sites remain unknown due to the lack of imaging tools to achieve simultaneous detection of these two substances, making it challenging to implement real-time imaging and precise tracking. Herein, we report an activity-based two-photon fluorescent probe, TPSP-1, for the cascade detection of H2S and NAT2 in living liver cells. Continuous conversion from TPSP-1 to TPSP-3 was achieved in liver cells and tissues. Significantly, leveraging the outstanding optical properties of this two-photon fluorescent probe, TPSP-1, has been effectively used to identify pathological tissue samples directly from clinical liver cancer patients. This work provides us with this novel sensing and two-photon imaging probe, which can be used as a powerful tool to study the physiological functions of H2S and NAT2 and will help facilitate rapid and accurate diagnosis and therapeutic evaluation of hepatocellular carcinoma.
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