Discovery of a Trisubstituted Thiochromenone as Fluorescent Probe for Detection of Hypochlorous Acid

Abstract Accurate detection of hypochlorous acid (HClO), which closely related to various pathophysiological processes, is pivotal for the diagnosis and treatment of related diseases. In this study, we developed 2,3,6‐tris(4‐diphenylamino)phenyl)‐4‐H‐thiochromen‐4‐one ( TTPA‐TC ) as a highly sensitive fluorescent probe for HClO sensing. The probe demonstrated excellent selectivity and repaid response kinetics (< 30 s) toward HClO with a large Stokes shift (184 nm) and a low detection limit (0.223 µM). The TTPA‐TC also exhibits aggregation‐induced emission (AIE) characteristics with intense yellow fluorescence emission. Furthermore, bioimaging experiments showed that its capability for real‐time monitoring of HClO dynamics in live RAW264.7 cells.