Detection and screening of organophosphate esters in infant formula from Shanghai, China: distribution characteristics and risk evaluation

Organophosphate esters (OPEs) have raised great concerns in recent years. However, information regarding their occurrence in infant formula remains limited. Thus, thirty-two OPEs were measured in infant formula sold in Shanghai, China in 2023. The results showed that OPE occurrence in infant formula was widespread. The median concentrations of organophosphate diesters, organophosphate triesters, and total OPEs were 2.28, 5.20, and 8.63 ng/g, respectively. Tris(2-chloroisopropyl) phosphate (TCPP) showed the highest median concentration (1.95 ng/g), followed by triethyl phosphate, bis(1-chloro-2-propyl) phosphate (BCPP), tri-isobutyl phosphate, and triphenyl phosphate (0.532-0.581 ng/g). The dominant chloro-OPEs (TCPP and BCPP) were regional-specific. Compared to corresponding triesters, the diester concentrations were often lower, except for bis(2-butoxyethyl) phosphate and tributoxyethyl phosphate. Additionally, five novel OPEs with phenyl groups were identified, showing high detection frequencies and comparable concentrations to TCPP. Raw materials and food processing methods might affect individual OPEs. The estimated daily intakes (EDIs) ranged from 62.3 to 355 ng/kg bw/day. The highest EDI occurred in infants of 0-6 months of age but posed no obvious health risk for infants and toddlers. Further studies are still needed to evaluate the possible health implications arising from the novel OPEs and their metabolites, as well as the potentially synergistic effects.