Clog P‐Guided Development of Multi‐Colored Buffering Fluorescent Probes for Super‐Resolution Imaging of Lipid Droplet Dynamics

Abstract Super‐resolution fluorescence imaging of live cells increasingly demands fluorescent probes capable of multi‐color and long‐term dynamic imaging. Understanding the mechanisms of probe‐target recognition is essential for the engineered development of such probes. In this study, it is discovered that the molecular lipid solubility parameter, Clog P , determines the staining performance of fluorescent dyes on lipid droplets (LDs). Fluorescent dyes with Clog P values between 2.5 and 4 can form buffering pools outside LDs, replacing photobleached dyes within LDs to maintain constant fluorescence intensity in LDs, thereby enabling dynamic super‐resolution imaging of LDs. Guided by Clog P , four different colored buffering LD probes spanning the visible light spectrum have been developed. Using Structured Illumination Microscopy (SIM), the role of LD dynamics have been tracked during cellular ferroptosis with the secretion, storage, and degradation of overexpressed ACSL3 proteins. It is found that LDs serve as storage sites for these proteins through membrane fusion, and further degrade overexpressed proteins via interactions with organelles like lysosomes or through lipophagy, thereby maintaining cellular homeostasis.