TCR beta Chain Repertoire Characteristic between Healthy Human CD4+ and CD8+ T Cells

T cell is vital in the adaptive immune system, which relays on TCR to recognize and defend against infection and tumors. T cells are mainly divided into well-known CD4+ and CD8+ T cells, which can recognize short peptide antigens presented by MHC class II and MHC class I respectively in humoral and cell-mediated immunity. Due to the HLA diversity and restriction with peptides complexation, TCRs are quite diverse and complicated. To better elucidate the TCR in humans, this study shows the difference between the TCR Repertoire in CD4+ and CD8+ T Cells from 30 healthy donors. The result showed count, clonality, diversity, frequency, and VDJ usage in CD4+ and CD8+ TCR-β repertoire is different, but CDR3 length is not. The Common Clone Cluster result showed repertoire showed that CD4+ and CD8+ TCR repertoire is connected separately between the bodies, which is odd considering the HLA diversity. More knowledge about TCR makes more opportunities for immunotherapy. The TCR repertoire is still a myth for discovery.