化学
脂肪酶
蛋氨酸
立体化学
有机化学
酶
生物化学
氨基酸
作者
Xiaojun Li,Li Qi,Yang Lee,Liqin Huang,Chenchen Peng,Jingwu Zheng
出处
期刊:Chirality
[Wiley]
日期:2024-01-29
卷期号:36 (2)
摘要
Abstract In this study, lipase‐catalyzed resolution of N‐acetyl‐DL‐methionine methyl ester (N‐Ac‐DL‐MetOMe) was evaluated. A lipase from Brucella thiophenivorans was prone to exhibit high activity and excellent enantioselectivity toward N‐Ac‐DL‐MetOMe to produce the key chiral intermediate N‐acetyl‐L‐methionine methyl ester (N‐Ac‐L‐MetOMe). The results showed that the enzymatic reaction was carried out in 100 g/L racemic substrate for 2 h, the conversion reached 51.3%, the enantiomeric excess value N‐Ac‐L‐MetOMe exceeded 99%, and the enantiomeric ratio value >200. Therefore, the lipase from B. thiophenivorans has potential prospects for the resolution of N‐Ac‐DL‐MetOMe to produce the important intermediate N‐Ac‐L‐MetOMe.
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