小桶
白血病
生物
淋巴瘤
免疫学
外周血单个核细胞
基因
内源性逆转录病毒
T细胞白血病
免疫系统
基因表达谱
淋巴细胞
基因表达
病毒学
基因组
转录组
遗传学
体外
作者
Tianfu Li,Kun Qian,Jingwan Han,Yongjian Liu,Lei Jia,Xiaolin Wang,Tianyi Li,Bohan Zhang,Jingyun Li,Hanping Li,Liping Dou,Lin Li
标识
DOI:10.1089/aid.2023.0037
摘要
Haematological malignant tumors (HMTs) are serious diseases that threaten human health and life with high mortality. Therefore, it is necessary to develop novel strategies for diagnosis and treatment. Human endogenous retroviruses (HERVs) have recently attracted increasing attention as potential targets for cancer diagnosis and therapy. In this study, we explored the association between HERV-K expression levels and HMTs development. Clinical data and peripheral blood samples were collected from 236 leukemia, 384 lymphoma patients and 69 healthy controls. Quantitative PCR was used to detect the expression of HERV-K gag, pol and env genes in peripheral blood mononuclear cells (PBMCs) or different cell subpopulations. Differently expressed HERV-K genes were further tested by using deep sequencing method, and further analyzed with Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment. B-cell- and T-cell-related cytokines in patients were also detected by ELISA. The results showed that the expression levels of the HERV-K gag, pol and env genes in patients were significantly higher than in healthy controls. There was a correlation between the expression level of HERV-K and the clinicopathological parameters of leukemia patients. HERV-K expression was increased in the B lymphocyte of leukemia and lymphoma patients, but not in the T cells or neutrophils. The GO and KEGG analyses showed that abnormal expression of the HERV-K locus in patients affected immune regulation. The analysis of cytokines proved that the B-cell-related cytokines, including IL-1β, IL-2, IL-4, IL-6, IL-10, TNF-α, and IFN-γ, were significantly decreased in patients, while the T-cell-related cytokines, including IL-3, IL-12, and TNF-β, were not significantly changed. In conclusion, HERV-K genes might participate in the occurrence and development of leukemia and lymphoma, and might be biomarkers for the detection or evaluation of leukemia and lymphoma.
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