糖肽
大肠杆菌
微生物学
沙门氏菌
肠炎沙门氏菌
化学
细菌
肠杆菌科
生物化学
生物
抗生素
基因
遗传学
作者
Baichong Yang,Xuemei Zhang,Xiaolan Bao,Le Ying,Jing Zhang,Shuntang Guo
标识
DOI:10.1016/j.foodres.2008.04.009
摘要
Glycopeptide, a compound utilized as a medicinal ingredient, was prepared through size exclusion chromatography following the alcalase digestion of soybean β-conglycinin. In vitro, experiments were conducted by using the LoVo (human colon adenocarcinoma) cell line to confirm if soybean glycopeptides, when applied to enteropathogens (Escherichia coli serotype O114, O111, Salmonella typhimurium, S. enteritidis), could produce an anti-adhesive effect on human intestinal cells. The results indicated that 0.5 mg mL−1 and 1.0 mg mL−1 of soybean glycopeptide can significantly inhibit the adhesion of the four bacteria strains to LoVo cells (S. typhimurium P < 0.05; S. enteritidis, E. coli O114 and E. coli O111 P < 0.01). The assay of lactate dehydrogenase (LDH) release revealed that soybean glycopeptide was effective in preventing the damage of the LoVo cells plasma membrane caused by bacterial infection. Soybean glycopeptide did not show inhibitory effects on bacterial growth in vitro. It was assumed that mannose substructure of soybean glycopeptide recognized by the bacteria block the adhesion of bacterial to LoVo cells. Based on these properties, soybean glycopeptide has the potential to be an effective ingredient with a protective effect against E. coli and Salmonellae infection.
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