生物
染色质免疫沉淀
表观遗传学
转录因子
计算生物学
表观遗传学
遗传学
染色质
DNA甲基化
DNA结合位点
芯片排序
基因
发起人
基因表达
染色质重塑
作者
Rasika Mundade,Hatice Gülçin Özer,Han Wang,Lakshmi Prabhu,Tao Lü
出处
期刊:Cell Cycle
[Informa]
日期:2014-09-17
卷期号:13 (18): 2847-2852
被引量:114
标识
DOI:10.4161/15384101.2014.949201
摘要
Many biologically significant processes, such as cell differentiation and cell cycle progression, gene transcription and DNA replication, chromosome stability and epigenetic silencing etc. depend on the crucial interactions between cellular proteins and DNA. Chromatin immunoprecipitation (ChIP) is an important experimental technique for studying interactions between specific proteins and DNA in the cell and determining their localization on a specific genomic locus. In recent years, the combination of ChIP with second generation DNA-sequencing technology (ChIP-seq) allows precise genomic functional assay. This review addresses the important applications of ChIP-seq with an emphasis on its role in genome-wide mapping of transcription factor binding sites, the revelation of underlying molecular mechanisms of differential gene regulation that are governed by specific transcription factors, and the identification of epigenetic marks. Furthermore, we also describe the ChIP-seq data analysis workflow and a perspective for the exciting potential advancement of ChIP-seq technology in the future.
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