体内
串联质谱法
化学
化学成分
质谱法
药理学
色谱法
体外
液相色谱-质谱法
传统医学
生物化学
生物
生物技术
医学
作者
Guifang Feng,Shizhe Li,Shu Liu,Fengrui Song,Zifeng Pi,Zhongfan Liu
标识
DOI:10.1021/acs.jafc.8b02753
摘要
Poria cocos are extensively used as nutritious food, dietary supplements, and oriental medicine in Asia. However, the effect substances are still not very clear. In this study, a targeted screening approach was developed to systematically identify absorbed constituents of Poria cocos in vivo using ultrahigh performance liquid chromatography tandem mass spectrometry combined with UNIFI software. First, incubation reactions in vitro with rat intestinal microflora and rat liver microsomes were conducted to sum up metabolic rules of main constituents. Second, the absorbed constituents in vivo were picked out and identified based on the results of metabolic study in vitro. Finally, the absorbed active constituents in the treatment of Alzheimer's disease were screened by targeted network pharmacology analysis. A total of 62 absorbed prototypes and 59 metabolites were identified and characterized in dosed plasma. Thirty potential active constituents were screened, and 86 drug-targets shared by absorbed constituents and Alzheimer's disease were discovered by targeted network pharmacology analysis. In general, this proposed targeted strategy comprehensively provides new insight for active ingredients of Poria cocos.
科研通智能强力驱动
Strongly Powered by AbleSci AI